5-Amino-1MQ

5-Amino-1MQ is a small synthetic molecule, not a peptide. In research, it targets NNMT, an enzyme that plays a role in metabolism and in the conversion of nicotinamide, a building block related to NAD+.

Mechanism of action under investigation

NNMT is an enzyme involved in metabolic processes; research suggests that increased activity of this enzyme is linked to fat metabolism. In experiments, 5-Amino-1MQ inhibits this enzyme, enabling researchers to study how this affects energy balance and the availability of NAD+-related building blocks. It therefore serves as a tool for unravelling the role of NNMT in metabolism.

This description relates to the mechanism of action as studied in research and does not constitute evidence of efficacy in humans.

The fact that 5-Amino-1MQ is a small molecule makes it an attractive research tool: researchers can use it to study the role of NNMT without the complexity of a larger protein. Furthermore, its link to NAD+ metabolism places it within a broad and topical field of research concerning metabolism and ageing. As such, 5-Amino-1MQ remains a useful tool for investigating fundamental metabolic questions.

5-Amino-1MQ is a relatively new research compound, best known for its use in preclinical studies of metabolism and body composition. It is used as a tool to investigate the role of NNMT.

• Research focus: inhibition of the enzyme NNMT.
• Research focus: fat metabolism and body composition (preclinical).
• Research focus: the relationship with NAD+-related building blocks.

Status and context

5-Amino-1MQ has not been approved as a medicinal product and research into it is largely at the preclinical stage. It is supplied exclusively as research material. As it is a relatively new compound, a pure starting material and careful documentation are particularly important for reliable, traceable results. In metabolic research, it is sometimes studied alongside NAD+.

As NNMT is present in various tissues and is associated with multiple metabolic processes, a selective tool such as 5-Amino-1MQ is valuable for distinguishing between cause and effect. As with many new compounds, most of the knowledge comes from animal and cell models, which requires critical interpretation and a pure starting material. Furthermore, the combination of metabolism and NAD+ makes the subject relevant to broader longevity research.

5-Amino-1MQ is supplied as a lyophilised powder and reconstituted in the laboratory using bacteriostatic water. The steps below describe only the laboratory procedures for research purposes.

1. 1Allow the vial and the bacteriostatic water to reach room temperature.
2. 2Wipe the rubber stoppers of both vials with an alcohol wipe.
3. 3Allow the bacteriostatic water to run slowly down the side of the vial; do not direct it straight onto the powder.
4. 4Gently swirl the vial until everything has dissolved; do not shake.
5. 5Store the solution in the fridge and label it with the date and batch number.

You can find supplies such as bacteriostatic water, insulin syringes and alcohol wipes under ‘Lab & Accessories’. This is a laboratory instruction and does not constitute dosage advice for consumption.

Working with a small molecule

Calculate the concentration accurately and record the method, concentration and batch number for each experiment. Prepare only the amount you need in the short term and store the rest in the fridge straight away.

Standardise your procedures and ensure that preparations remain consistent, so that measurements remain comparable across experiments. Check the solution for clarity before use. Only prepare what you need in the short term.

Store 5-Amino-1MQ in lyophilised (powder) form in a cool, dark place for maximum stability.

Practical storage tips

• Powder form: freezer (-20 °C) for long-term storage; fridge (2–8 °C) for short-term storage.
• After reconstitution: store refrigerated (2–8 °C) and use within a few weeks.
• Avoid direct light, heat and repeated temperature changes.
• Write the reconstitution date and batch number on the vial.

Consistent, cold and dark storage ensures reliable, reproducible measurement results across experiments.

Consistent, cold storage ensures reliable, reproducible measurement results. Check the solution for clarity before use.